国内团队：富含氨基酸的饮食或可通过调节肠菌预防结肠炎

mSystems[IF:7.324]

① 为评估富含氨基酸元素的饮食（ED）对慢性结肠炎的影响，ED/正常喂食小鼠2周后给予DSS处理（诱导肠炎模型）；② ED可抑制NF-kB通路和MAPK信号激活、减少下游炎症因子释放、增加粘蛋白表达，进而改善小鼠结肠炎；③ ED喂养降低了粘液降解细菌丰度（如AKK和拟杆菌）及粘液溶解酶活性，可能和结肠蛋白发酵减少有关；④ 移植ED喂养小鼠的肠道菌群可重塑受体小鼠肠道微生态，减轻肠道炎症和对粘液层的侵蚀，但ED干预未能缓解无菌小鼠的结肠炎。

An Elemental Diet Enriched in Amino Acids Alters the Gut Microbial Community and Prevents Colonic Mucus Degradation in Mice with Colitis
12-05, doi: 10.1128/msystems.00883-22

【主编评语】膳食氨基酸或完整蛋白质在结肠炎进展中的作用仍有争议，涉及肠道菌群的机制尚不清楚。近日，南开大学医学院王硕及团队在mSystems发表最新研究，发现富含氨基酸元素的饮食（ED）可抑制NF-kB通路和MAPK信号激活、减少下游炎症因子释放、增加粘蛋白表达，进而改善小鼠结肠炎。利用粪菌移植和无菌小鼠实验，揭示出肠道菌群在调节小鼠结肠炎中发挥关键作用，即ED处理减少了粘液降解细菌丰度，从而保护粘液层免受微生物入侵和降解。

An Elemental Diet Enriched in Amino Acids Alters the Gut Microbial Community and Prevents Colonic Mucus Degradation in Mice with Colitis

富含氨基酸的元素饮食可改变肠道微生物群落并防止结肠炎小鼠的结肠粘液降解

10.1128/msystems.00883-22

12-05, Article

Abstract:

The role of dietary amino acids or intact proteins in the progression of colitis remains controversial, and the mechanism involving gut microbes is unclear. Here, we investigated the effects of an elemental diet (ED) enriched in amino acids and a polymeric diet enriched in intact protein on the pathogenesis of dextran sulfate sodium (DSS)-induced colitis in mice. Our results showed that the ED induced remission of colitis in mice. Notably, ED treatment reduced the abundance of the mucolytic bacteria Akkermansia and Bacteroides, which was attributed to decreased colonic protein fermentation. Consistently, the activities of mucolytic enzymes were decreased, leading to protection against mucus layer degradation and microbial invasion. Fecal microbiota transplantation from ED-fed mice reshaped microbial ecology and alleviated intestinal inflammation in recipient mice. The ED failed to induce remission of colitis in pseudogermfree mice. Together, our results demonstrate the critical role of the gut microbiota in the prevention of colitis by an ED.

First Authors:
Bowei Zhang,Congying Zhao

Correspondence Authors:
Shuo Wang

All Authors:
Bowei Zhang,Congying Zhao,Xuejiao Zhang,Xiang Li,Yunhui Zhang,Xiaoxia Liu,Jia Yin,Xinyang Li,Jin Wang,Shuo Wang