张和平+孙志宏Nature子刊：内蒙古人肠道菌群高质量基因组集合

1、利用PromethION和HiSeq平台对180份粪便样本进行宏基因组测序，生成3.7Tbps三代和20.1Tbps二代数据；

2、通过评估在宏基因组中组装出的Probio-M8基因组，发现足量二代测序对保证组装质量至关重要；

3、构建了内蒙古人肠道基因组数据集（IMGG），包括802个环状和5927个高质量基因组，显著提升了二代测序中易被遗漏区域的分析性能；

4、报道了430种未培养物种的rRNA基因拷贝数，超过12000种未知肠道前噬菌体，以及插入序列在肠道细菌中的分布。

A high-quality genome compendium of the human gut microbiome of Inner Mongolians

内蒙古人肠道菌群高质量基因组简编

10.1038/s41564-022-01270-1

01-05, Article

Abstract:
Metagenome-based resources have revealed the diversity and function of the human gut microbiome, but further understanding is limited by insufficient genome quality and a lack of samples from typically understudied populations. Here, we used hybrid long-read PromethION and short-read HiSeq sequencing to characterize the fecal microbiota of 60 Inner Mongolian individuals (n=180 samples over three time points), who were part of a probiotic yogurt intervention trial. We present the Inner Mongolian Gut Genome (IMGG) catalog, comprising 802 closed and 5,927 high-quality metagenome-assembled genomes (MAGs). This approach achieved high genome continuity, substantially increased the resolution of genomic elements, including ribosomal RNA operons (rrns), metabolic gene clusters (MGCs), prophages, and insertion sequences (IS). Particularly, we report the rrn copy number for uncultured species, over 12,000 previously undescribed gut prophages, and the distribution of IS elements across gut bacteria. Overall, these data provide a high-quality, large-scale resource for studying the human gut microbiota.

First Authors:
Hao Jin,Keyu Quan,Qiuwen He

Correspondence Authors:
Heping Zhang,Zhihong Sun

All Authors:
Hao Jin,Keyu Quan,Qiuwen He,Lai-Yu Kwok,Teng Ma,Yalin Li,Feiyan Zhao,Lijun You,Heping Zhang,Zhihong Sun