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内蒙古农业大学:干酪乳杆菌Zhang在肠道内的“变化”

时间:2021-07-09 08:06来源:热心肠日报 作者:热心肠日报 浏览次数:
  1.  基于宏基因组和宏转录组探究干酪乳杆菌Zhang(LcZ)在体内外的基因表达差异,以及肠道共生菌群对LcZ的应答;

  2.  LcZ进入人体肠道后,其复制和转录增加,而肠内原有的干酪乳杆菌菌株不受影响;

  3.  进入肠道后,LcZ约39%的mRNA和45%的sRNA被抑制,而rli28c sRNA被激活,其在体外抑制了LcZ生长和乳酸产生

  4.  LcZ的ABC转运蛋白和氨基酸代谢基因、糖和SCFA代谢基因,分别在摄入第14和28天被激活;

  5.  人肠道菌群对LcZ的应答是有限且异质的。
 
Metagenomic and metatranscriptomic profiling of Lactobacillus casei Zhang in the human gut
人肠道中干酪乳杆菌Zhang的宏基因组和宏转录组分析
10.1038/s41522-021-00227-2
07-01, Article

Abstract:
Little is known about the replication and dynamic transcription of probiotics during their "passenger" journey in the human GI tract, which has therefore limited the understanding of their probiotic mechanisms. Here, metagenomic and metatranscriptomic sequencing was used to expose the in vivo expression patterns of the probiotic Lactobacillus casei Zhang (LcZ), which was compared with its in vitro growth transcriptomes, as well as the dynamics of the indigenous microbiome response to probiotic consumption. Extraction of the strain-specific reads revealed that replication and transcripts from the ingested LcZ were increased, while those from the resident L. casei strains remained unchanged. Mapping of all sequencing reads to LcZ genome showed that gene expression in vitro and in vivo differed dramatically. Approximately 39% of mRNAs and 45% of sRNAs of LcZ well-expressed were repressed after ingestion into human gut. The expression of ABC transporter genes and amino acid metabolism genes was induced at day 14 of ingestion, and genes for sugar and SCFA metabolism were activated at day 28 of ingestion. Expression of rli28c sRNA with peaked expression during the in vitro stationary phase was also activated in the human gut; this sRNA repressed LcZ growth and lactic acid production in vitro. However, the response of the human gut microbiome to LcZ was limited and heterogeneous. These findings implicate the ingested probiotic has to change its transcription patterns to survive and adapt in the human gut, and the time-dependent activation patterns indicate highly dynamic cross-talk between the probiotic and human gut microbes.

First Authors:
Jicheng Wang,Jiachao Zhang
Correspondence Authors:
Zhihong Sun
All Authors:
Jicheng Wang,Jiachao Zhang,Wenjun Liu,Heping Zhang,Zhihong Sun




 
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